Sciatic nerve regeneration after traumatic injury using magnetic targeted adipose-derived mesenchymal stem cells

Traumatic peripheral nerve injuries constitute a huge concern to public health. Nerve damage leads decrease or even loss of mobility the innervated area. Adult stem cell therapies have shown some encouraging results and been identified as promising treatment candidates for regeneration. A major obstacle that approach is securing sufficient number cells at injured site produce measurable therapeutic effects. The present work tackles this issue demonstrates enhanced regeneration ability promoted by magnetic targeted therapy in an vivo Wallerian degeneration model. To end, adipose-derived mesenchymal (AdMSC) were loaded with citric acid coated superparamagnetic iron oxide nanoparticles (SPIONs), systemically transplanted magnetically recruited sciatic nerve. AdMSC arrival was significantly increased using targeting their beneficial effects surpassed regenerative properties stand-alone therapy. AdMSC-SPIONs group showed partially conserved structure many intact myelinated axons. Also, very remarkable restoration myelin basic protein organization, indicative remyelination, observed. This resulted improvement conduction, demonstrating functional recovery. In summary, our demonstrate assisted delivery AdMSC, non-invasive non-traumatic method, highly strategy promote recruitment after traumatic injury. Last but not least, validate exceeding previous reports less complex models through vitro ex vivo. health concern. They can lead areas. Due pathophysiology, current pharmacological surgical approaches are only effective. Cell-based emerged useful tool achieve full tissue However, bottleneck enough sites. Therefore, proposal combining biological (adipose derived cells) nanotechnological strategies (magnetic targeting) great relevance, reporting first experiments involving “magnetic cell” Using improved, fostering remyelination